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Neural activity in human eyeblink conditioning: an optically pumped magnetometer-based MEG study

Lin, C.-H. S.; Tierney, T. M.; Mellor, S.; ONeill, G. C.; Bestmann, S.; Barnes, G. R.; Miall, R. C.

2022-10-21 neuroscience
10.1101/2022.10.19.510727 bioRxiv
Show abstract

There is a profound lack of electrophysiological data from the cerebellum in humans, as compared to animals, because the pervading perception is that it is difficult to record human cerebellar activity non-invasively using magnetoencephalography (MEG) or electroencephalography (EEG). We used on-scalp MEG based on optically pumped magnetometers (OP-MEG) to detect learning related cerebellar signals in a classical eyeblink conditioning paradigm. In four healthy human adults, we observed unconditioned stimulus-related evoked responses locating in the cerebellum. These evoked responses diminished during the acquisition of conditioned responses, which corresponds with previous observed changes of Purkinje cell activities in animals. We also observed evoked responses immediately before conditioned blinks in 3 out of 4 participants, which were also located in the cerebellum. We discuss the potential nature of these cerebellar evoked responses and future applications of OP-MEG to the studies of human cerebellar physiology.

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