Reclassification of probiotic Lactobacillus acidophilus NCIMB 30184 as Lactobacillus helveticus and Lactobacillus casei NCIMB 30185 as Lacticaseibacillus paracasei
Rodenes, A.; Casinos Ramo, B.; Alvarez Perez, B.; Martinez Blanch, J. F.; Vijayakumar, V.; Superti, G.; Day, R.; Chenoll Cuadros, E. M.
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The strains NCIMB 30184 and NCIMB 30185, originally designated as Lactobacillus acidophilus and Lactobacillus casei respectively, have been studied in published pre-clinical and clinical studies. Their nucleotide sequences of the 16S rRNA were compared with public databases and were preliminarily found to have the highest similarity with Lactobacillus helveticus and Lacticaseibacillus paracasei species respectively. Subsequently, total 16S and 23S rRNA genes were compared with their most similar taxa, and the preliminary identification as Lactobacillus helveticus and Lacticaseibacillus paracasei were confirmed.
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