Mechanobiology of Fibroblast Activation in Skin Grafting
Hong, Y.; Peng, X.; Yu, H.; Jafari, M.; Shakiba, D.; Sandler, J.; Pryse, K.; Elson, E.; Genin, G.; Alisafaei, F.
Show abstract
Mechanical stretching of living tissues can activate long-lived changes in tissue cells such as fibroblasts, increasing their contractility and initiating phenotypic transformations. Increased mechanical stimulus typically leads to monotonically increasing activation of fibroblasts cultured in 2D, but activation levels are difficult to predict for cells in 3D fibrous tissues, leading to variable outcomes in procedures such as skin grafting. Here we report that the source of this variation is cell-extracellular matrix (ECM) interactions and their variation with the duration and magnitude of applied stretch, and present a model that can predict the degree to which stretch will either increase or decrease long-term activation levels of fibroblasts cultured within a stretched, three-dimensional collagen matrix. Combining experimental and mathematical approaches across multiple scales, we show that the viscoplasticity of the ECM regulates this nonmonotonic, long-term cell activation. Results demonstrate that feedback between cell and ECM determines how cells retain memory of mechanical stretch.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Directed biomechanical compressive forces enhance fusion efficiency in model placental trophoblast cultures 95%
- Tissue-engineered collagenous fibrous cap models to systematically elucidate atherosclerotic plaque rupture. 94%
- Substrate stiffness regulates triple-negative breast cancer signaling through CXCR4 receptor dynamics 93%
Similar papers in this journal
Similar papers in this journal
- Extracellular Matrix Physical Properties Regulate Cancer Cell Morphological Transitions in 3D Hydrogel Microtissues 94%
- Dynamic remodeling of fiber networks with stiff inclusions under compressive loading 94%
- Multicellular dynamics on structured surfaces: Stress concentration is a key to controlling complex microtissue morphology on engineered scaffolds 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.