Single-cell profiling of complex plant responses to Pseudomonas syringae infection
Zhu, J.; Lolle, S.; Tang, A.; Guel, B.; Kvikto, B.; Cole, B.; Coaker, G.
Show abstract
Plant response to pathogen infection varies within a leaf, yet this heterogeneity is not well resolved. We exposed Arabidopsis to Pseudomonas syringae or mock treatment and profiled >11,000 individual cells using single-cell RNA sequencing. Integrative analysis of cell populations from both treatments identified distinct pathogen responsive cell clusters exhibiting transcriptional responses ranging from immunity to susceptibility. Pseudotime analyses through pathogen infection revealed a continuum of disease progression from an immune to susceptible state. Confocal imaging of promoter reporter lines for transcripts enriched in immune cell clusters expressed surrounding substomatal cavities colonized or in close proximity to bacterial colonies, suggesting cells within immune clusters represent sites of early pathogen invasion. Susceptibility clusters exhibited more general localization and were highly induced at later stages of infection. Overall, our work uncovers cellular heterogeneity within an infected leaf and provides unique insight into plant differential response to infection at a single-cell level.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Establishing Physalis as a new Solanaceae model system enables genetic reevaluation of the inflated calyx syndrome 95%
- De novo stem cell establishment in meristems requires repression of organ boundary cell fate 95%
- The blast effector Pwl2 is a virulence factor that modifies the cellular localisation of host protein HIPP43 to suppress immunity. 95%
Similar papers in this journal
- Interfamily co-transfer of sensor and helper NLRs extends immune receptor functionality between angiosperms 96%
- TIR domains of plant immune receptors are 2',3'-cAMP/cGMP synthetases mediating cell death 93%
- Systematic identification and characterization of novel genes in the regulation and biogenesis of photosynthetic machinery 93%
Similar papers in this journal
Similar papers in this journal
- Cell-type specific gating of gene regulatory modules as a hallmark of early immune responses in Arabidopsis leaves 98%
- Warm temperature modifies cell fates to reduce stomata production in Arabidopsis 95%
- A conserved gene regulatory network controls root epidermal cell patterning in superrosid species 95%
Similar papers in this journal
- Light prevents pathogen-induced aqueous microenvironments via potentiation of salicylic acid signaling 96%
- A fungal secondary metabolism gene cluster enables mutualist-pathogen transition in root endophyte Colletotrichum tofieldiae 96%
- Humidity-driven ABA depletion determines plant-pathogen competition for leaf water 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.