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A hybrid reconstruction of the physical model with the deep-learning that improves structured illumination microscopy

Wang, J.; Fan, J.; Zhou, B.; Huang, X.; Chen, L.

2022-10-07 bioengineering
10.1101/2022.10.04.510914 bioRxiv
Show abstract

In handling raw images with low signal-to-noise (SNR) ratios, conventional algorithms of structured illumination microscopy are prone to artifacts, while deep-learning-based (DL) algorithms may lead to degradation and hallucinations. We propose a hybrid that combines the physical inversion model with a Total Deep Variation regularization. In super-resolving from low SNR images such as actin filaments, our method outperforms conventional or DL methods in suppressing artifacts and hallucinations while maintaining resolutions.

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