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Simultaneous multicolor fluorescence imaging using duplication-based PSF engineering

Van den Eynde, R.; Hertel, F.; Krajnik, B.; Hugelier, S.; Auer, A.; Schlichthaerle, T.; Jungmann, R.; Leutenegger, M.; Vandenberg, W.; Dedecker, P.

2022-10-05 biophysics
10.1101/2022.10.04.510770 bioRxiv
Show abstract

We present a way to encode more information in fluorescence imaging by splitting the emission into copies of the original point-spread function (PSF), which offers broadband operation and compatibility with other PSF engineering modalities and existing analysis tools. We demonstrate the approach using the Circulator, an add-on that encodes the fluorophore emission band into the PSF, enabling simultaneous multicolor super-resolution and single-molecule microscopy using essentially the full field of view.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.