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Highly Sensitive and Specific Amyloid-β Oligomers Detection Using CRISPR-Cas12a Probe System aided by Graphene Oxide for Early Diagnosis of Alzheimer's Diseases

Tang, J.; Zhu, Z.; Yang, C.

2022-09-28 biochemistry
10.1101/2022.09.27.509482 bioRxiv
Show abstract

Alzheimers disease (AD) is highly age-specific that results in cognitive incapability including dementia. It is proposed that AD is caused by the dysfunctional gene producing excessive amyloid-{beta} protein that eventually results in aggregated form of amyloid-{beta} Oligomers (A{beta}O). We couple the CRISPR-Cas12a system with a specially designed single-stranded DNA (ssDNA) aptamer that specifically forms a hybrid complex with A{beta}O. We exploit the aptamer as the substrate for the crRNA-aptamer duplex due to its high flexibility, sensitivity, and selectivity. We further adopted the graphene oxide (GO) fluorescent system to couple with our CRISPR-Cas12a probe system. Our coupled probe system can effectively detect the presence of A{beta}O by quantitatively returning fluorescent signals with high specificity and accuracy.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.