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The p-rpS6-zone delineates wounding response and the healing process

Ring, N. A. R.; Dworak, H.; Bachmann, B.; Schaedl, B.; Valdivieso, K.; Rozmaric, T.; Heimel, P.; Schuetzenberger, K.; Leinfellner, G.; Ferguson, J.; Drechsler, S.; Mildner, M.; Schosserer, M.; Slezak, P.; Meyuhas, O.; Gruber, F.; Grillari, J.; Redl, H.; Ogrodnik, M.

2022-09-28 physiology
10.1101/2022.09.27.507541 bioRxiv
Show abstract

It is unknown what the spatial boundaries of tissue response to wounding are. Here we show that in mammals the ribosomal protein S6 (rpS6) is phosphorylated in response to skin injury forming a zone of activation surrounding the region of the initial insult. This p-rpS6-zone forms within minutes after wounding and is present until healing is complete. The zone encapsulates markers of the healing process, including proliferation, senescence, and angiogenesis in wounded skin. A mouse model unable to phosphorylate rpS6 shows an initial acceleration of wound closure, but results in disrupted healing. Finally, the p-rpS6-zone accurately reports on the status of dermal vasculature and the effectiveness of healing. In summary, the zone divides an otherwise homogenous tissue into regions with distinct properties.

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