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Reduced mitochondrial transcription sensitizes acute myeloid leukemia cells to BCL-2 inhibition

Arabanian, L. S.; Adamsson, J.; Unger, A.; Di Lucrezia, R.; Bergbrede, T.; Ashouri, A.; Larsson, E.; Nussbaumer, P.; Klebl, B. M.; Palmqvist, L.; Gustafsson, C. M.

2022-09-25 cancer biology
10.1101/2022.09.23.509145 bioRxiv
Show abstract

Overcoming drug-resistance and the subsequent relapse that often occurs with monotherapy is crucial in the treatment of acute myeloid leukemia. We here demonstrate that therapy-resistant leukemia initiating cells can be targeted using a novel inhibitor of mitochondrial transcription (IMT). The compound inhibits mitochondrial RNA polymerase activity and sensitizes the resistant population to the induction of apoptosis. In vitro studies on acute myeloid leukemia cells demonstrate that IMT prevents cell proliferation, and together with a selective BCL-2 inhibitor, venetoclax, induces apoptosis and suppress oxidative phosphorylation (OXPHOS) synergistically. AML mouse models treated with IMT in combination with venetoclax show prolonged survival in venetoclax-resistant models. Our findings suggest that certain therapy-resistant leukemia cell populations display a unique dependency on mitochondrial transcription and can be targeted with IMT.

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