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Sequence-based Optimized Chaos Game Representation and Deep Learning for Peptide/Protein Classification

Huang, B.; Zhang, E.; Chaudhari, R.; Gimperlein, H.

2022-09-13 bioinformatics
10.1101/2022.09.10.507145 bioRxiv
Show abstract

As an effective graphical representation method for 1D sequence (e.g., text), Chaos Game Representation (CGR) has been frequently combined with deep learning (DL) for biological analysis. In this study, we developed a unique approach to encode peptide/protein sequences into CGR images for classification. To this end, we designed a novel energy function and enhanced the encoder quality by constructing a Supervised Autoencoders (SAE) neural network. CGR was used to represent the amino acid sequences and such representation was optimized based on the latent variables with SAE. To assess the effectiveness of our new representation scheme, we further employed convolutional neural network (CNN) to build models to study hemolytic/non-hemolytic peptides and the susceptibility/resistance of HIV protease mutants to approved drugs. Comparisons were also conducted with other published methods, and our approach demonstrated superior performance. Supplementary informationavailable online

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