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Designing Of Custom Barcodes For Sequencing On The Mgi Platform

Bulusheva, I.; Shmitko, A.; Vasiliadis, I.; Suchalko, O.; Syrko, D.; Belova, V.; Korostin, D.

2022-09-08 genomics
10.1101/2022.09.07.506907 bioRxiv
Show abstract

The recently launched MGI platform (DNBSEQ-G50, -G400 and -T7 sequencers) has been gaining popularity for next-generation sequencing. However, the barcode set for library preparation provided by the manufacturer has certain limitations on the number of samples that can be sequenced at a time and the compatibility of barcodes from different or incomplete sets as well as a ratio between the samples. In this paper, we present a protocol for designing custom barcodes expanding the pre-existing barcode set and demonstrate its performance on the MGI Tech Co. Ltd. (China) machines. We developed a universal "quad method" which allows for simultaneous sequencing of any number of samples up to 252 per lane which is multiple of 4 or 4n+2. Here, we describe this method, its analysis, verification, and integration into the sequencing as well as its validation for sequencing using the DNBSEQ G-400 machine.

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