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Effects of a Ketogenic and Low Fat Diet on the Human Metabolome, Microbiome and Food-ome in Adults at Risk for Alzheimer's Disease

Dilmore, A. H.; Martino, C.; Neth, B. J.; West, K. A.; Zemlin, J.; Rahman, G.; Panitchpakdi, M.; Meehan, M. J.; Weldon, K. C.; Blach, C.; Schimmel, L.; Kaddurah-Daouk, R.; Dorrestein, P.; Knight, R.; Craft, S.; Alzheimer's Gut Microbiome Project Consortium,

2022-08-31 neurology
10.1101/2022.08.30.22279087 medRxiv
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STRUCTURED ABSTRACTO_ST_ABSINTRODUCTIONC_ST_ABSThe ketogenic diet (KD) is an intriguing candidate for neuroprotection in Alzheimers disease (AD) given its protective effects against metabolic dysregulation and seizures. The diets neuroprotective effects have been shown to be gut microbiome-dependent in mice; thus we examined KD-induced changes in the gut microbiome and metabolome in patients at-risk for AD. METHODSWe compared the low-carbohydrate modified Mediterranean Ketogenic Diet (MMKD) to the low-fat American Heart Association Diet (AHAD) in adults with mild cognitive impairment (MCI) and control participants. We collected stool samples for shotgun metagenomics and untargeted metabolomics at five timepoints to interrogate the microbiome and metabolome. RESULTSIndividuals with MCI on the MMKD had lower levels of GABA-producing microbes Alistipes sp. CAG:514 and GABA, and higher levels of GABA-regulating microbes Akkermansia muciniphila. DISCUSSIONWe hypothesize that MMKD protects individuals with MCI in part via influencing on GABA levels and gut-transit time.

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