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Super-resolution imaging reveals dynamic skeletal cytoophidia

Fang, Y.; Li, Y.-L.; Li, X.-M.; Liu, J.-L.

2022-08-29 biophysics
10.1101/2022.08.29.505636 bioRxiv
Show abstract

CTP synthase (CTPS) can form filamentous structures termed cytoophidia in cells from all three domains of life. In order to study the mesoscale structure of cytoophidia, we perform fluorescence recovery after photobleaching (FRAP) and stimulated emission depletion (STED) microscopy in human cells. By using EGFP dimeric tag, as a tool to explore the physical properties of cytoophidia, we find that cytoophidia are dynamic and reticular. The reticular structure of CTPS cytoophidia may provide space for other components such as IMPDH. In addition, we observe CTPS granules with tentacles.

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