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STAT3 restricts prostate cancer metastasis and antiandrogen resistance by controlling LKB1/CREB signaling pathway

Pencik, J.; Philippe, C.; Schlederer, M.; Pecoraro, M.; Grund-Groeschke, S.; Li, W. J.; Tracz, A.; Heidegger, I.; Lagger, S.; Trachtova, K.; Oberhuber, M.; Heitzer, E.; Osman, O.; Neubauer, H. A.; Wingelhofer, B.; Orlova, A.; Witzeneder, N.; Dillinger, T.; Redl, E.; Greiner, G.; D'Andrea, D.; Oestman, J. R.; Tangermann, S.; Hermanova, I.; Schaefer, G.; Varady, A.; Horvath, J.; Stoiber, D.; Malcolm, T. I.; Turner, S. D.; Parkes, E. E.; Hantusch, B.; Egger, G.; Rose-John, S.; Poli, V.; Jain, S.; Armstrong, C. W. D.; Hoermann, G.; Goffin, V.; Aberger, F.; Moriggl, R.; Carracedo, A.; McKinney, C.;

2022-08-26 cancer biology
10.1101/2022.08.25.504915 bioRxiv
Show abstract

Prostate cancer (PCa) lethality is driven by its progression to a metastatic castration-resistant state, yet the signaling mechanisms underlying metastatic spread remain unknown. Here we show that STAT3 converges with the LKB1/mTORC1 and CREB to control metastatic disease in PCa mouse models. Unexpectedly, STAT3 was found to be upregulated in diabetic PCa patients undergoing metformin therapy with a concomitant reduction in mTORC1 expression. In preclinical mouse models of PCa, genetic ablation or activation of STAT3 had opposing effects on LKB1/AMPK/mTORC1- dependent tumorigenesis. Using genetic and pharmacological approaches, we identified LKB1 as a direct STAT3 target while repressing CREB. Furthermore, PCa patients with high CREB expression had inferior clinical outcome with significantly increased risk of disease and metastatic recurrence. We observe that castration state lowers STAT3 abundance and increases AR and CREB levels, leading to castration-resistant PCa (CRPC). Our findings revealed that STAT3 controls mTORC1 and CREB in metastatic disease, suggesting CREB as a promising target for lethal CRPC.

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