Back

RGG-motif proteins regulate mRNA translation upon genotoxic stress

Roy, R.; Mohanan, G.; Malka-Mahieu, H.; Biswas, A.; Labbe, C.; Vagner, S.; Rajyaguru, P. I.

2022-07-13 cell biology
10.1101/2022.07.12.499753 bioRxiv
Show abstract

Genotoxic stress response (GSR) mediated by mRNA translation and decay regulation remains poorly explored. Here, we identify a unique role of yeast RGG-motif protein Scd6 and its human ortholog LSM14A in mRNA translation control upon hydroxyurea (HU)-mediated GSR. Scd6/LSM14A, but not all tested RGG-containing proteins, localize to HU-induced cytoplasmic puncta in an RGG-dependent manner. The absence of Scd6 increases HU tolerance but sensitizes the cells to HU upon overexpression of SRS2, a known dampener of the DNA- damage response. Scd6 binds SRS2 mRNA to repress its translation in cytoplasmic granules upon HU stress. Scd6-SRS2 interaction is modulated by arginine methylation (AM) and the LSm-domain, which acts as a cis-regulator of Scd6 AM. Polysome-profiling experiments indicate that LSM14A regulates the translation of NHEJ factor mRNAs such as LIG4 (DNL4 homolog) and RTEL1 (SRS2 functional homolog), and the NHEJ activity in response to HU. Overall, this report unveils the role of AM and Scd6/LSM14A in the GSR by determining the translation status of specific mRNAs.

Matching journals

The top 7 journals account for 50% of the predicted probability mass.

1
Nature Communications
○ 5641 papers in training set
Top 15%
12.3%
Similar papers in this journal
2
Journal of Biological Chemistry
○ 690 papers in training set
Top 1.0%
8.6%
Similar papers in this journal
3
eLife
● 5828 papers in training set
Top 15%
7.6%
Similar papers in this journal
4
Nucleic Acids Research
◐ 1281 papers in training set
Top 3%
6.5%
Similar papers in this journal
5
Molecular and Cellular Biology
○ 47 papers in training set
Top 0.1%
6.5%
Similar papers in this journal
6
Life Science Alliance
● 285 papers in training set
Top 0.4%
5.4%
7
RNA
● 189 papers in training set
Top 0.4%
5.4%
50% of probability mass above
8
Cell Reports
○ 1498 papers in training set
Top 7%
5.3%
9
The EMBO Journal
○ 309 papers in training set
Top 0.7%
5.3%
10
PLOS Genetics
● 862 papers in training set
Top 3%
5.0%
11
Molecular Cell
○ 350 papers in training set
Top 2%
3.9%
12
Journal of Cell Biology
● 392 papers in training set
Top 2%
3.1%
13
iScience
○ 1154 papers in training set
Top 8%
3.1%
14
Journal of Cell Science
● 393 papers in training set
Top 2%
2.7%
15
Scientific Reports
○ 3612 papers in training set
Top 38%
2.7%
16
Proceedings of the National Academy of Sciences
● 2444 papers in training set
Top 31%
1.5%
17
Science Advances
● 1243 papers in training set
Top 24%
1.3%
18
Communications Biology
○ 993 papers in training set
Top 23%
1.1%
19
Biology Open
● 156 papers in training set
Top 3%
1.0%
20
EMBO Reports
○ 263 papers in training set
Top 6%
1.0%
21
Cellular and Molecular Life Sciences
○ 96 papers in training set
Top 2%
0.8%

"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.