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Increased glycolysis and functional mitochondria fuel acinar-ductal metaplasia in the pancreas

Neuss, T.; Wirges, N.; Chen, M.-C.; Usluer, S.; Oellinger, R.; Lier, S.; Dudek, M.; Madl, T.; Jastroch, M.; Steiger, K.; Schmitz, W.; Einwaechter, H.; Schmid, R. M.

2022-06-29 cancer biology
10.1101/2022.06.27.495427 bioRxiv
Show abstract

Reprogramming of the cellular metabolism is a hallmark of pancreatic cancer, yet it remains unclear at what stage during carcinogenesis it occurs. Here, we investigated the metabolic requirements for acinar-to-ductal metaplasia (ADM), the first step in pancreatic carcinogenesis. We detected increased glycolytic marker expression in human ADM suggesting that a metabolic switch occurs during ADM formation. We report that this switch was similarly required for ADM formation in different oncogenic mouse models (KRAS, PI3K, and MEK1) and in ligand-induced ADM in mouse wild-type acini. In addition, we show that a functional electron transport chain (ETC), but not mitochondrial ATP production, was essential to ADM formation. We conclude that the ETC provides NAD+ for the de novo synthesis of serine from glycolysis intermediates. Our findings demonstrate that metabolic programming is essential for the initiation of pancreatic carcinogenesis and thus identifies potential targets for metabolic intervention.

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