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From sequence to Boltzmann weighted ensemble of structures with AlphaFold2-RAVE

Vani, B. P.; Aranganathan, A.; Wang, D.; Tiwary, P.

2022-05-26 biophysics
10.1101/2022.05.25.493365 bioRxiv
Show abstract

While AlphaFold2 is rapidly being adopted as a new standard in protein structure predictions, it is limited to single structure prediction. This can be insufficient for the inherently dynamic world of biomolecules. Even with recent modifications towards conformational diversity, AlphaFold2 is devoid of providing thermodynamically ranked conformations. AlphaFold2-RAVE is an efficient protocol using the structural outputs from AlphaFold2 as initializations for AI augmented molecular dynamics. These simulations result in Boltzmann ranked ensembles, which we demonstrate on different proteins.

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