Impact of Shelterin Complex on Telomere Accessibility
Shiekh, S.; Jack, A.; Mustafa, G.; Kodikara, S. G.; Gyawali, P.; Hoque, M. E.; Yildiz, A.; Balci, H.
Show abstract
Shelterin plays critical roles in maintaining and protecting telomeres by regulating access of various physiological agents to telomeric DNA. We present single molecule measurements investigating the impact of the POT1 and a four-component shelterin complex on the accessibility of human telomeric DNA overhangs with physiologically relevant lengths (28-150 nt), which to our knowledge is the first direct approach to measure this effect on such telomeric constructs. To quantify telomere accessibility, we monitored transient binding events of a short peptide nucleic acid (PNA) probe that is complementary to telomeric overhangs using FRET-PAINT. Although POT1 has a mild G-quadruplex unfolding activity, it reduced accessibility of the PNA probe by [~]2.5 fold, indicating that POT1 effectively binds to and protects otherwise exposed telomeric sequences. In comparison, a four-component shelterin reduced the accessibility of telomeric overhangs by [~]5-fold. This enhanced protection suggests shelterin restructures the region between single and double stranded telomere, which is otherwise the most accessible part of the overhang, by a synergistic cooperation of shelterin components located on single and double stranded telomere.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Base-pair resolution analysis of the effect of supercoiling on DNA flexibility and major groove recognition by triplex-forming oligonucleotides 95%
- Assembly mechanism of the AIM2 inflammasome sensorrevealed by single-molecule analysis 95%
- Single-molecule analysis reveals TDG exhibits multiple modes of linear diffusion to process 5-formylcytosine. 95%
Similar papers in this journal
- DNA-sequence dependent positioning of the linker histone in a nucleosome: a single-pair FRET study 94%
- Characterization of human XPD helicase activity with Single Molecule Magnetic Tweezers 93%
- Single-molecule FRET and tracking of transfected biomolecules: multi-dimensional protein dynamics in living cells 92%
Similar papers in this journal
- Single molecule microscopy to profile the effect of zinc status of transcription factor dynamics 93%
- Sequential deregulation of histone marks, chromatin accessibility and gene expression in response to PROTAC-induced degradation of ASH2L 93%
- Comparison of retinol binding protein 1 with cone specific G-protein as putative effector molecules in cryptochrome signalling 92%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.