Analysis of the Drosophila Ajuba LIM protein defines functions for distinct LIM domains
Rauskolb, C.; Han, A.; Kirichenko, E.; Ibar, C.; Irvine, K.
Show abstract
The Ajuba LIM protein Jub mediates regulation of Hippo signaling by cytoskeletal tension through interaction with the kinase Warts, and also participates in feedback regulation of junctional tension through regulation of the cytohesin Steppke. To investigate how Jub interacts with and regulates its distinct partners, we investigated the ability of Jub proteins missing different combinations of its three LIM domains to rescue jub phenotypes and to interact with -catenin, Warts and Steppke. Multiple regions of Jub contribute to its ability to bind -catenin and to localize to adherens junctions in Drosophila wing imaginal discs. Co-immunoprecipitation experiments in cultured cells identified a specific requirement for LIM2 for binding to Warts. However, in vivo, both LIM1 and LIM2, but not LIM3, were required for regulation of wing growth, Yorkie activity, and Warts localization. Conversely, LIM2 and LIM3, but not LIM1, were required for regulation of cell shape and Steppke localization in vivo, and for maximal Steppke binding in co-immunoprecipitation experiments. These observations identify distinct functions for the different LIM domains of Jub.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- DAnkrd49 and Bdbt act via Casein kinase Iϵ to regulate planar polarity in Drosophila 97%
- Filamin protects myofibrils from contractile damage through changes in its mechanosensory region 96%
- Intimate functional interactions between TGS1 and the Smn complex revealed by an analysis of the Drosophila eye development 95%
Similar papers in this journal
- Exploring the evolution and function of Canoes intrinsically disordered region in linking cell-cell junctions to the cytoskeleton during embryonic morphogenesis 95%
- Inducible degradation of the Drosophila Mediator subunit Med19 reveals its role in regulating developmental but not constitutively-expressed genes 95%
- Linking Expression and Function of Drosophila Type-I TGF-b Receptor Baboon Isoforms: Multiple Roles of BaboA Isoform in Shaping of the Adult Central Nervous System 94%
Similar papers in this journal
- The Drosophila Tumour Suppressor Lgl and Vap33 activate the Hippo pathway by a dual mechanism, involving RtGEF/Git/Arf79F and inhibition of the V-ATPase. 96%
- Mask, the Drosophila Ankyrin Repeat and KH domain-containing protein, regulates microtubule dynamics 94%
- The dual Ras Association (RA) Domains of Drosophila Canoe have differential roles in linking cell junctions to the cytoskeleton during morphogenesis 93%
Similar papers in this journal
- Reduced function of the RNA export factor, Nxt1, in Drosophila causes muscle degeneration and lowers expression of genes with long introns and circular RNA 94%
- Pvr and downstream signaling factors are required for spreading of Drosophila hemocytes at larval wound sites 94%
- Multiple requirements for Rab GTPases in the development of Drosophila tracheal dorsal branches and terminal cells 93%
Similar papers in this journal
- Interdomain interactions regulate the localization of a lipid transfer protein at ER-PM contact sites 94%
- The lipid transfer function of RDGB at ER-PM contact sites is regulated by multiple interdomain interactions. 94%
- A kinase translocation reporter reveals real-time dynamics of ERK activity in Drosophila 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.