Comparison of simple RNA extraction methods for molecular diagnosis of hepatitis C virus in plasma
Hongjaisee, S.; Jabjainai, Y.; Sakset, S.; Preechasuth, K.; Ngo-Giang-Huong, N.; Khamduang, W.
Show abstract
We evaluated the efficacy of four simple RNA extraction methods for the detection of hepatitis C virus (HCV) in plasma samples: silica-membrane-based, magnetic beads-based, boiling with Diethyl Pyrocarbonate (DEPC)-treated distilled water or a commercial lysis buffer. HCV RNA was detected using both real time reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription loop mediated isothermal amplification (RT-LAMP). The magnetic beads-based extraction can be used as an alternative RNA extraction method for on-site HCV detection. Boiling with DEPC-treated distilled water was not appropriate for low HCV load samples and boiling with a lysis buffer was not recommended.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- SARS-CoV-2 detection in multi-sample pools in a real pandemic scenario: a screening strategy of choice for active surveillance 95%
- Independent Assessment of a Point of Care HCV RNA Test by Laboratory Analytical Testing and a Prospective Field Study in the U.S 95%
- Seroprevalence and molecular characterization of viral hepatitis and HIV co-infection in the Central African Republic 95%
Similar papers in this journal
- Evaluation of commercial qPCR kits for detection of SARS-CoV-2 in pooled samples 95%
- Evaluation of VIDAS® diagnostic assay prototypes detecting dengue virus NS1 antigen and anti-dengue virus IgM and IgG antibodies 94%
- A simple, cost-effective and extraction-free molecular diagnostic test for sickle cell disease in noninvasive buccal swab specimen for a limited-resource setting 92%
Similar papers in this journal
- Evaluation of two RT-PCR techniques for SARS-CoV-2 RNA detection in serum for microbiological diagnosis 96%
- Performance evaluation of Xpert HBV viral load (VL) assay: Point-of-care molecular test to strengthen and decentralize management of chronic hepatitis B (CHB) infection 95%
- Diagnostic comparison of three fully automated chemiluminescent immunoassay platforms for the detection of SARS-CoV-2 antibodies 94%
Similar papers in this journal
- Performance of COVIDSeq and Swift normalase amplicon SARS-CoV-2 panels for SARS-CoV-2 Genomes Sequencing: Practical Guide and Combining FASTQ Strategy 93%
- Test Agreement Between Roche Cobas 6800 and Cepheid GeneXpert Xpress SARS-CoV-2 Assays at High Cycle Threshold Ranges 93%
- Validation and multi-site deployment of a lyophilized qRT-PCR reagent for the molecular diagnosis of avian influenza and rabies in Sub-Saharan African regions 92%
Similar papers in this journal
- Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis 94%
- A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR 94%
- Ivermectin reduces coronavirus infection in vivo: a mouse experimental model 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.