Post-transcriptional control of SRSF9 promotes the epithelial-to-mesenchymal transition (EMT) in colorectal cancer cells
Rao, C.; Svoboda, R. A.; Southekal, S.; Vieira, H. M.; Huisman, D. H.; Chatterjee, D.; Guda, C.; Fisher, K. W.; Anczukow, O.; Lewis, R. E.
Show abstract
In human colorectal cancer (CRC) cells the Raf/MEK/ERK scaffold Kinase Suppressor of Ras 1 (KSR1)-dependent signaling is required for the epithelial-to-mesenchymal transition (EMT)-like phenotype. Here we show that KSR1 promotes the association of differentially spliced mRNA bearing recognition sites for the Serine/Arginine-Rich (SR) splicing factor SRSF9. CRISPR/Cas9 disruption of KSR1 destabilizes SRSF9 protein, which interacts preferentially with mRNA encoding Epithelial Stromal Interaction 1 (EPSTI1). EPSTI1 protein mediates Ras and KSR1-dependent induction of EMT. Analysis of EPSTI1 splice variants reveals that inclusion of exon 8 is critical to the ability of EPSTI1 to promote the E-to N-cadherin switch and CRC cell motile and invasive behavior. These data reveal a mechanism in CRC cells in which Ras-induced and KSR1-dependent signaling affects pre-mRNA splicing to control behaviors critical to cancer cell dissemination and metastasis.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Systematic mapping of rRNA 2'-O methylation during frog development and involvement of the methyltransferase Fibrillarin in eye and craniofacial development in Xenopus laevis 93%
- The MOV10 RNA helicase is a dosage-dependent host restriction factor for LINE1 retrotransposition in mice 93%
- mRNA 5' terminal sequences drive 200-fold differences in expression through effects on synthesis, translation and decay 93%
Similar papers in this journal
- The Exon Junction Complex Core Represses Caner-specific Mature mRNA Re-splicing: A Potential Key Role in Terminating Splicing 94%
- Kinase Suppressor of RAS 1 (KSR1) maintains the transformed phenotype of BRAFV600E mutant human melanoma cells 93%
- mTOR-driven widespread exon skipping renders multifaceted gene regulation and proteome complexity 93%
Similar papers in this journal
- A 36-base hairpin within lncRNA DRAIC, which is modulated by alternative splicing, interacts with the IKKα coiled-coil domain and inhibits NF-κB and tumor cell phenotypes 96%
- The Cancer Testis Antigen Testis Specific Serine Kinase 6 (TSSK6) is abnormally expressed in colorectal cancer and promotes oncogenic behaviors 94%
- UHMK1 is a novel splicing regulatory kinase 94%
Similar papers in this journal
- Common tissue-specific expressions and regulatory mechanisms of c-KIT isoforms with and without GNNK and GNSK sequences across five mammals 93%
- Autocrine Signaling by Receptor Tyrosine Kinases in Urothelial Carcinoma of the Bladder 93%
- The nuclear receptor NR4A1 is regulated by SUMO modification to induce autophagic cell death 93%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.