Composition can buffer protein dynamics within liquid-like condensates.
Jelenic, S.; Bindics, J.; Czermak, P.; Pillai, B. R.; Ruer, M.; Holehouse, A. S.; Saha, S.
Show abstract
Most non-membrane-bound compartments in cells that form via phase separation have complex composition. While phase separation of individual proteins that form these compartments is well-documented, the mechanisms that modulate dynamics of individual proteins in multicomponent systems remain unclear. Here, we used in vitro reconstitution and in vivo experiments to investigate how the dynamics of a scaffold protein PGL-3 is regulated within the liquid-like P granule compartment in C. elegans. Using mutational and biophysical perturbations, we generated PGL-3 constructs that form condensates in vitro with widely varying dynamics. Using these PGL-3 constructs, we show that introducing other P granule components buffers against change of dynamics within liquid-like condensates. This dynamics-buffering effect is mediated by weak interactions among two or more components. Such dynamics-buffering may contribute to robust functional output of cellular liquid-like compartments.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Treacle's ability to form liquid-like phase condensates is essential for nucleolar fibrillar center assembly, efficient rRNA transcription and processing, and rRNA gene repair 96%
- The intrinsically disordered cytoplasmic tail of a dendrite branching receptor uses two distinct mechanisms to regulate the actin cytoskeleton 96%
- The MIDAS domain of AAA mechanoenzyme Mdn1 forms catch bonds with two different substrates 96%
Similar papers in this journal
- Structural insights into p300 regulation and acetylation-dependent genome organisation 95%
- β-Catenin condensation facilitates clustering of the cadherin/catenin complex and formation of nascent cell-cell junctions 95%
- Structural analysis of Red1 as a conserved scaffold of the RNA-targeting MTREC/PAXT complex 95%
Similar papers in this journal
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.