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NOMe-HiC: joint profiling of genetic variants, DNA methylation, chromatin accessibility, and 3D genome in the same DNA molecule

Fu, H.; Zheng, H.; Chen, X.; Weirauch, M. T.; Muglia, L. J.; Wang, L.; Liu, Y.

2023-02-28 genomics
10.1101/2022.03.29.486102 bioRxiv
Show abstract

Cis-regulatory elements coordinate the regulation of their targeted genes expression. However, the joint measurement of cis-regulatory elements activities and their interactions in spatial proximity is limited by the current sequencing approaches. We describe a method, NOMe-HiC, which simultaneously captures single nucleotide polymorphisms, DNA methylation, chromatin accessibility (GpC methyltransferase footprints), and chromosome conformation changes from the same DNA molecule, together with the transcriptome, in a single assay. NOMe-HiC shows high concordance with state-of-the-art mono-omic assays across different molecular measurements and reveals coordinated chromatin accessibility at distal genomic segments in spatial proximity and novel types of long-range allele-specific chromatin accessibility.

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