Zfp281 inhibits the pluripotent-to-totipotent state transition in mouse embryonic stem cells
Wen, X.; Lin, Z.; Wu, H.; Fu, X.
Show abstract
The cell-fate transition between pluripotent and totipotent states determines embryonic development and the first cell-lineage segregation. However, limited by the scarcity of totipotent embryos, regulators on this transition remain largely elusive. A novel model to study the transition has been recently established, named the 2-cell-like (2C-like) model. The 2C-like cells are rare totipotent-like cells in the mouse embryonic stem cell (mESC) culture. Pluripotent mESCs can spontaneously transit into and out of the 2C-like state. We previously dissected the transcriptional roadmap of the transition. In this study, we revealed that Zfp281 is a novel regulator for the pluripotent-to-totipotent transition in mESCs. Zfp281 is a transcriptional factor involved in the cell-fate transition. Our study shows that Zfp281 represses transcripts upregulated during the 2C-like transition via Tet1 and consequentially inhibits mESCs from transiting into the 2C-like state. Interestingly, we found that the inhibitory effect of Zfp281 on the 2C-like transition leads to an impaired 2C-like-transition ability in primed-state mESCs. Altogether, our study reveals a novel mediator for the pluripotent-to-totipotent state transition in mESCs and provides insights into the dynamic transcriptional control of the transition.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes 93%
- ScRNA-seq and scATAC-seq reveal that sertoli cell mediate spermatogenesis disorders through stage-specific communications in non-obstructive azoospermia 93%
- Obox4 promotes zygotic genome activation upon loss of Dux 93%
Similar papers in this journal
- Importance of transcript variants in transcriptome analyses 93%
- Impaired p53-mediated DNA damage response contributes to microcephaly in Nijmegen Breakage Syndrome patient-derived cerebral organoids 93%
- Comparative analyses of gene expression in common marmoset and human pluripotent stem cells (PSCs) identify factors enhancing homologous recombination efficiency in the HPRT locus of human PSCs 92%
Similar papers in this journal
- Toti: an integrated multi-omics database to decipher the epigenetic regulation of gene expression in totipotent stem cells 96%
- Canonical Wnt pathway controls mESCs self-renewal through inhibition of spontaneous differentiation via β-catenin/TCF/LEF functions 94%
- Single Cell Analysis Reveals Partial Reactivation of X-chromosome Instead of chromosome-wide dampening in Naïve Human Pluripotent Stem Cells 93%
Similar papers in this journal
- The pluripotent stem cell-specific transcript ESRG is dispensable for human pluripotency 96%
- Integrative genomic analysis of early neurogenesis reveals a temporal genetic program for differentiation and specification of preplate and Cajal-Retzius neurons 94%
- The RNA helicase DDX6 controls early mouse embryogenesis by repressing aberrant inhibition of BMP signaling through miRNA-mediated gene silencing 94%