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Hypoxia shapes the immune landscape in lung injury promoting inflammation persistence

Mirchandani, A. S.; Jenkins, S. J.; Bain, C. C.; Lawson, H.; Coelho, P.; Murphy, F.; Griffith, D.; Zhang, A.; Sanchez-Garcia, M. A.; Reyes, L.; Morrison, T.; Arienti, S.; Sadiku, P.; Watts, E.; Dickinson, R.; Clark, S.; Ly, T.; Lewis, D.; Kelly, V.; Spanos, C.; Musgrave, K.; Delaney, L.; Harper, I.; Scott, J.; Parkinson, N.; Rostron, A.; Baillie, J. K.; Clohisey, S. M. R.; Pridans, C.; Campana, L.; Starkey, P.; Simpson, A. J.; Dockrell, D.; Schwarze, J.; Hirani, N.; Ratcliffe, P.; Pugh, C.; Kranc, K.; Forbes, S.; Whyte, M.; Walmsley, S.

2022-03-12 cell biology
10.1101/2022.03.11.483935 bioRxiv
Show abstract

Acute Respiratory Distress Syndrome (ARDS), an often-fatal complication of pulmonary or systemic inflammation, has no cure. Hypoxemia is a defining feature, yet its impact on inflammation is often neglected. Patients with ARDS are monocytopenic early in the onset of the disease. Endotoxin or Streptococcus pneumoniae acute lung injury (ALI) in the context of hypoxia replicates this finding, through hypoxia-driven suppression of type I interferon signalling. This results in failed lung monocyte-derived interstitial macrophages (IM) niche expansion and unchecked neutrophilic inflammation. Administration of colony stimulating factor 1 (CSF1) rescues the monocytopenia, alters the circulating classical monocyte phenotype in hypoxic endotoxin-driven ALI and enables lung IM population expansion, thus limiting lung injury in endotoxin- and virally-induced hypoxic ALI. Hypoxia directly alters immune dynamics to the detriment of the host and manipulation of this aberrant response offers new therapeutic strategies for ARDS.

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