Comparing lipid remodeling in mouse adipose and liver tissue with quantitative Raman microscopy
Paul, A.; Chanclon, B.; Brännmark, C.; Wittung-Stafshede, P.; Olofsson, C. S.; Asterholm, I. W.; Parekh, S. H.
Show abstract
Brown adipose tissue (BAT) consists of highly metabolically active adipocytes that catabolize nutrients to produce heat. Playing an active role in triacylglycerol (TAG) clearance, research has shown that dietary fatty acids can modulate the TAG chemistry deposition in BAT after weeks-long dietary intervention, similar to what has been shown in white adipose tissue (WAT). Our objective was to compare the influence of sustained, non-chronic dietary intervention (a one-week interval) on WAT and BAT lipid metabolism and deposition in situ. We use quantitative, label-free chemical microscopy to show that one week of HFD intervention results in dramatically larger lipid droplet (LD) growth in BAT (and liver) compared to LD growth in inguinal WAT (IWAT). Moreover, BAT showed lipid remodeling as increased unsaturated TAGs in LDs, resembling the dietary lipid composition, while WAT (and liver) did not show lipid remodeling on this time scale. Concurrently, expression of genes involved in lipid metabolism, particularly desaturases, was reduced in BAT and liver from HFD-fed mice after one week. Our data show that BAT lipid chemistry remodels exceptionally fast to dietary lipid intervention compared WAT, which further points towards a role in TAG clearance.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Loss of Carnitine Palmitoyltransferase 1a Reduces Docosahexaenoic Acid-Containing Phospholipids and Drives Sexually Dimorphic Liver Disease in Mice 95%
- Active integrins regulate white adipose tissue insulin sensitivity and brown fat thermogenesis 95%
- Dietary isoleucine content defines the metabolic and molecular response to a Western diet 95%
Similar papers in this journal
- ABHD4 regulates adipocyte differentiation in vitro but does not affect adipose tissue lipid metabolism in mice 96%
- Quantifying acyl-chain diversity in isobaric compound lipids containing monomethyl branched-chain fatty acids 95%
- Automated preparation of plasma lipids, metabolites, and proteins for LC/MS-based analysis of a high-fat diet in mice 94%
Similar papers in this journal
- Metabolomic and transcriptomic remodeling of bone marrow myeloid cells in response to maternal obesity 96%
- ANGPTL4 from adipose, but not liver, is responsible for regulating plasma triglyceride partitioning 95%
- Adipose depot-specific upregulation of Ucp1 or mitochondrial oxidative complex proteins are early consequences of genetic insulin reduction in mice 95%
Similar papers in this journal
- Resolvin E1 derived from eicosapentaenoic acid prevents hyperinsulinemia and hyperglycemia in a host genetic manner 95%
- Sustained anti-obesity effects of life-style change and anti-inflammatory interventions after conditional inactivation of the activin receptor ALK7 95%
- Creld2 function during unfolded protein response is essential for liver metabolism homeostasis 94%
Similar papers in this journal
- Chronic exposure to IL6 leads to deregulation of glycolysis and fat accumulation in the zebrafish liver 94%
- Human adipocyte differentiation and composition of disease relevant lipids are regulated by miR-221-3p 94%
- A diet-independent zebrafish model for NAFLD recapitulates patient lipid profiles and offers a system for small molecule screening 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.