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Synchronic resisto-genesis effects identify most prevalent mechanism in Acinetobacter baumannii

Alaei, N.; Bahador, A.; Alaei, A.

2022-03-03 microbiology
10.1101/2022.03.03.482757 bioRxiv
Show abstract

Acinetobacter baumannii as deadliest infection agent to ICU patients challenge experimental treatment in lack of new antibiotic and non-optimal recommendations. Despite confirm importance of infectious ICU sources, IC2 strains, ISAba/blaOXA genes and colistin in adequate concentrations or combination therapy due to potential heteroresistance; this study reveals unusual hypersensitive colistin- and sensitive aminoglycosides-resistant strains using designed methods, antibio-dendrogram classification and a computational table. Accordingly, lipopolysaccharide loss-mediated penetration and upregulated AdeAB pump-mediated compounds exclusion, are found to be causes of these abnormalities. Consequently, these simultaneous resistances termed synchronic resisto-genesis effects, drive population and create abnormal multidrug-resistant strains; while, delay extensively drug-resistant phenotype. This phenomenon predicts the predominant determinant mechanisms and makes abnormal behavior comprehensible among antibiotic-phenotypic levels or in vitro and vivo observations. Therefore, this method provides preclinical information to address therapeutic resistance in future microbial or cancer cell studies, and improves drug choosing in combination therapies, like use of inhibitory drugs.

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