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Study on the usefulness of Direct Saliva sample Collection (DiSC) by polyester swab

Aoki, K.; Nagashima, M.; Kashiwagi, K.; Chiba, T.; Sadamasu, K.; Ishii, Y.; Tateda, K.

2022-02-28 infectious diseases
10.1101/2022.02.25.22271529 medRxiv
Show abstract

Saliva sample can be self-collected and used in testing of SARS-CoV-2 nucleic acid amplification tests (NAATs) test in Japan. However, this may have difficulty collecting a proper specimen when collecting for the first time. We compared 2 collection methods, conventional methods and Direct Saliva Sample Collection method (DiSC) from 44 asymptomatic or symptomatic individuals who were in quarantine in Toho university hospital. RT-PCR by DiSC method showed about 70 % positive percent agreement compared to RT-PCR by conventional methods. In addition, comparing RT-PCR and TMA by DiSC method, TMA showed about 90 % positive percent agreement compared to RT-PCR. DiSC method is easy to perform by every person, does not have complicated restrictions/instructions and can be used in RT-PCR and TMA. This method allows for ease of saliva collection in certain patient populations.

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