Brachypodium antifreeze protein gene products inhibit ice recrystallization, attenuate ice nucleation, and reduce immune response
Juurakko, C. L.; diCenzo, G. C.; Walker, V. K.
Show abstract
Antifreeze proteins (AFPs) from the model crop, Brachypodium distachyon, allow freeze survival and attenuate pathogen-mediated ice nucleation. Intriguingly, each Brachypodium AFP gene encodes two proteins, an autonomous AFP and a leucine-rich repeat (LRR). We present structural models suggesting that ice-binding motifs on the ~13 kDa AFPs can "spoil" nucleating arrays on the ~120 kDa bacterial ice nucleating proteins that form ice at high sub-zero temperatures, consistent with decreases in ice nucleating activity by lysates from wildtype compared to transgenic Brachypodium lines. Strikingly, the expression of Brachypodium LRRs in transgenic Arabidopsis inhibited an immune response to pathogen flagella peptides (flg22), with structural modelling suggesting this was due to affinity of the LRR domains to flg22. Thus, these genes play distinctive roles in connecting freeze survival and anti-pathogenic systems via their encoded proteins ability to adsorb to ice as well as attenuating bacterial ice nucleation and the host immune response.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Chemical inhibition of stomatal differentiation by perturbation of the master-regulatory bHLH heterodimer via an ACT-Like domain 94%
- The Botrytis cinerea Crh transglycosylae is a cytoplasmic effector triggering plant cell death and defense response 94%
- Design of diverse, functional mitochondrial targeting sequences across eukaryotic organisms using variational autoencoder 94%
Similar papers in this journal
- A deeply conserved protease, acylamino acid-releasing enzyme (AARE), acts in ageing in Physcomitrella and Arabidopsis 95%
- Structural and Functional Analyses Explain Pea KAI2 Receptor Diversity and Reveal Stereoselective Catalysis During Signal Perception 95%
- LPMO-oxidized cellulose oligosaccharides evoke immunity in Arabidopsis conferring resistance towards necrotrophic fungus B. cinerea 94%
Similar papers in this journal
- De novo designed ice-binding proteins from twist-constrained helices 96%
- Antifungal symbiotic peptide NCR044.1 exhibits unique structure and multi-faceted mechanisms of action that confer plant protection 95%
- S1 basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs 94%
Similar papers in this journal
Similar papers in this journal
- Pre-meiotic H1.1 degradation is essential for Arabidopsis gametogenesis 94%
- The Proxitome of Arabidopsis Processing Bodies Reveals a Condensate-Membrane Interface Instructing Actin-Driven Directional Growth 94%
- ROS production by localized SCHENGEN receptor module drives lignification at subcellular precision 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.