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Viral Cultures for Assessing Airborne Transmission of SARS-CoV-2: a Systematic Review Protocol

Heneghan, C. J.; Spencer, E. A.; Brassey, J.; Rosca, E. C.; Pluddemann, A.; Evans, D. H.; Conly, J. M.; Maltoni, S.; Jefferson, T.; ONAKPOYA, I. J.

2022-01-30 intensive care and critical care medicine
10.1101/2022.01.28.22270021 medRxiv
Show abstract

This is a protocol for a systematic review that aims to evaluate the role of viral cultures for assessing airborne transmission of SARS-CoV-2. The review will address the following research questions: Are airborne samples infectious? If so, what proportion are infectious, and what is the distance and duration of infectiousness in the air? What is the relationship between infectiousness and airborne PCR cycle threshold (Ct)? Is there evidence of a chain of transmission that establishes an actual instance of airborne transmission of SARS-CoV-2? What circumstances might facilitate infectious viruses being airborne over long distances? We will search LitCovid, medRxiv, Google Scholar, and the WHO Covid-19 database to identify relevant studies. We will include studies reporting airborne transmission attempting viral culture or serial qRT-PCR with or without genomic sequencing. Predictive or modelling studies will be excluded. We will assess the quality of included studies using previously published criteria.

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