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An ancient role for the Hippo pathway in axis formation and morphogenesis

Brooun, M.; Salvenmoser, W.; Dana, C.; Sudol, M.; Steele, R.; Hobmayer, B.; McNeill, H.

2022-01-20 developmental biology
10.1101/2022.01.19.476962 bioRxiv
Show abstract

How did cells of early metazoan organisms first organize themselves to form a body axis? The canonical Wnt pathway has been shown to be sufficient for induction of axis in Cnidaria, a sister group to Bilateria, and is important in bilaterian axis formation. Here, we provide experimental evidence that in cnidarian Hydra the Hippo pathway regulates the formation of a new axis during budding upstream of the Wnt pathway. The target of Hippo pathway, the transcriptional co-activator YAP, inhibits the initiation of budding in Hydra, and is regulated by Hydra LATS. In addition, we show functions of Hippo pathway in regulation of actin organization and cell proliferation in Hydra. We hypothesize that Hippo pathway served as a link between continuous cell division, cell density and axis formation early in metazoan evolution.

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