Identification and Age-dependent Increase of Platelet Biased Human Hematopoietic Stem Cells
Aksoz, M.; Gafencu, G.-A.; Stoilova, B. S.; Buono, M.; Meng, Y.; Jakobsen, N. A.; Metzner, M.; Clark, S.-A.; Beveridge, R.; Thongjuea, S.; Vyas, P.; Nerlov, C.
Show abstract
Hematopoietic stem cells (HSC) reconstitute multi-lineage human hematopoiesis after clinical bone marrow transplantation and are the cells-of-origin of hematological malignancies. Though HSC provide multi-lineage engraftment, individual murine HSCs are lineage-biased and contribute unequally to blood cell lineages. Now, by combining xenografting of molecularly barcoded adult human bone marrow (BM) HSCs and high-throughput single cell RNA sequencing we demonstrate that human individual BM HSCs are also functionally and transcriptionally lineage biased. Specifically, we identify platelet-biased and multi-lineage human HSCs. Quantitative comparison of transcriptomes from single HSCs from young, and aged, BM show that both the proportion of platelet-biased HSCs, and their level of transcriptional platelet priming, increases with age. Therefore, platelet-biased HSCs, as well as their increased prevalence and elevated transcriptional platelet priming during ageing, are conserved between human and murine hematopoiesis. One-Sentence SummaryIn vivo barcoding and single cell RNA sequencing identifies platelet-biased human bone marrow HSCs.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Resolving fate and transcriptome of hematopoietic stem cell clones 98%
- Hematopoietic stem cells fail to regenerate following inflammatory challenge. 96%
- Post-Transplant Administration of G-CSF Impedes Engraftment of Gene Edited Human Hematopoietic Stem Cells by Exacerbating the p53-Mediated DNA Damage Response 96%
Similar papers in this journal
- Clonal analysis of fetal hematopoietic stem/progenitor cell subsets reveals how post-transplantation capabilities are distributed 95%
- Developmental regulation of endothelial-to-hematopoietic transition from induced pluripotent stem cells 95%
- Developmental stage-specific changes in protein synthesis differentially sensitize hematopoietic stem cells and erythroid progenitors to impaired ribosome biogenesis 95%
Similar papers in this journal
- A single cell framework identifies functionally and molecularly distinct multipotent progenitors in adult human hematopoiesis 97%
- Distinct causes of three phenotypic hallmarks of hematopoietic aging 96%
- Concurrent stem- and lineage-affiliated chromatin programs precede hematopoietic lineage restriction 96%
Similar papers in this journal
- Multi-Modal Profiling of Human Fetal Liver-Derived Hematopoietic Stem Cells Reveals the Molecular Signature of Engraftment Potential 95%
- Engineered niches support the development of human dendritic cells in humanized mice 95%
- Inflammatory signals from fatty bone marrow supports the early stages of DNMT3a driven clonal hematopoiesis 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.