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Hypercapnia limits β-catenin mediated alveolar type 2 cell progenitor function by altering Wnt production from adjacent fibroblasts

Dada, L. A.; Welch, L. C.; Magnani, N. D.; Ren, Z.; Brazee, P. L.; Celli, D.; Flozak, A. S.; Weng, A.; Maciel-Herrerias, M.; Kryvenko, V.; Vadasz, I.; Runyan, C. E.; Abdala-Valencia, H.; Shigemura, M.; Casalino-Matsuda, S. M.; Misharin, A. V.; Budinger, G. R. S.; Gottardi, C. J.; Sznajder, J. I.

2022-01-13 cell biology
10.1101/2022.01.12.475264 bioRxiv
Show abstract

Persistent symptoms and radiographic abnormalities suggestive of failed lung repair are among the most common symptoms in patients with COVID-19 after hospital discharge. In mechanically ventilated patients with ARDS secondary to SARS-CoV-2 pneumonia, low tidal volumes to reduce ventilator-induced lung injury necessarily elevate blood CO2 levels, often leading to hypercapnia. The role of hypercapnia on lung repair after injury is not completely understood. Here, we show that hypercapnia limits {beta}-catenin signaling in alveolar type 2 (AT2) cells, leading to reduced proliferative capacity. Hypercapnia alters expression of major Wnts in PDGFRa+-fibroblasts from those maintaining AT2 progenitor activity and towards those that antagonize {beta}-catenin signaling and limit progenitor function. Activation of {beta}-catenin signaling in AT2 cells, rescues the inhibition AT2 proliferation induced by hypercapnia. Inhibition of AT2 proliferation in hypercapnic patients may contribute to impaired lung repair after injury, preventing sealing of the epithelial barrier, increasing lung flooding, ventilator dependency and mortality.

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