SOX17 Is Not Required for the Derivation and Maintenance of Mouse Extraembryonic Endoderm Stem Cell Lines
Dong, X.; Ding, A.; Lin, J.
Show abstract
Extraembryonic endoderm stem (XEN) cell lines can be derived and maintained in vitro and reflect the primitive endoderm cell lineage. SOX17 is thought to be required for the derivation and maintenance of mouse XEN cell lines. Here we have re-evaluated this requirement for SOX17. We derived multiple SOX17-deficient XEN cell lines from preimplantation embryos of a SOX17-Cre knockout strain and chemically converted multiple SOX17-deficient embryonic stem cell lines into XEN cell lines by transient culturing with retinoic acid and Activin A. We confirmed the XEN profile of SOX17-deficient cell lines by immunofluorescence with various markers, by NanoString gene expression analyses, and by their contribution to the extraembryonic endoderm of chimeric embryos produced by injecting these cells into blastocysts. Thus, SOX17 is not required for the derivation and maintenance of XEN cell lines.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Apoptosis, G1 phase stall and premature differentiation account for low chimeric competence of Human and rhesus monkey naive pluripotent stem cells 97%
- Arp2/3 complex activity is necessary for mouse ESC differentiation, times formative pluripotency, and enables lineage specification 96%
- Differential repression of Otx2 underlies the capacity of NANOG and ESRRB to induce germline entry 96%
Similar papers in this journal
- Rapid and robust directed differentiation of mouse epiblast stem cells into definitive endoderm and forebrain organoids 96%
- ETV4 and ETV5 Orchestrate FGF-Mediated Lineage Specification and Epiblast Maturation during Early Mouse Development 96%
- Fate specification triggers a positive feedback loop of TEAD-YAP and NANOG to promote epiblast formation in preimplantation embryos 96%
Similar papers in this journal
- Human spinal cord differentiation proceeds rapidly in vitro and only initially maintains differentiation pace in a heterologous environment 95%
- Asymmetric inheritance of centrosomes maintains stem cell properties in human neural progenitor cells 95%
- Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells 95%
Similar papers in this journal
- Pannexin 1 influences lineage specification of human iPSCs 94%
- Hindbrain rhombomere centers harbor a heterogenous population of dividing progenitors which rely on Notch-signaling 94%
- Canonical Hedgehog Signaling Controls Astral Microtubules and Mitotic Spindle Orientation in Neural Progenitors and iPSCs 94%
Similar papers in this journal
- Differential regulation of lineage commitment in human and mouse primed pluripotent stem cells by NuRD 95%
- Generation of Pathogenic TPP1 Mutations in Human Stem Cells as a Model for CLN2 Disease 93%
- Generation of human induced pluripotent stem cell (hiPSC) lines from patients with extreme high and low polygenic scores for QT interval 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.