TXNRD3 supports male fertility via the redox control of spermatogenesis
Dou, Q.; Turanov, A. A.; Mariotti, M.; Hwang, J. Y.; Wang, H.; Lee, S.-G.; Paulo, J. A.; Yim, S. H.; Gygi, S.; Chung, J.-J.; Gladyshev, V. N.
Show abstract
Thioredoxin/glutathione reductase (TGR, TXNRD3) is a thiol oxidoreductase of unknown function composed of thioredoxin reductase and glutaredoxin domains. This NADPH-dependent enzyme evolved by gene duplication within the Txnrd family, is expressed in the testes and can reduce both thioredoxin and glutathione in vitro. To characterize the function of TXNRD3 in vivo, we generated a strain of mice with the deletion of Txnrd3 gene. We show that Txnrd3 knockout mice are viable and without discernable gross phenotypes, but TXNRD3 deficiency leads to fertility impairment in male mice. Txnrd3 knockout animals exhibit a lower fertilization rate in vitro, a sperm movement phenotype and an altered redox status of thiols. Proteomic analyses revealed a broad range of substrates reduced by TXNRD3 during sperm maturation, presumably as a part of quality control. The results show that TXNRD3 plays a critical role in male reproduction via the thiol redox control of spermatogenesis.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Increased mitochondrial activity upon CatSper channel activation is required for sperm capacitation. 94%
- Peroxidasin protein expression and enzymatic activity in metastatic melanoma cell lines are associated with invasive potential 92%
- Disulfide-mediated tetramerization of TRAP1 fosters its antioxidant and pro-neoplastic activities. 92%
Similar papers in this journal
- Taf7L Regulates Early Stages Of Male Germ Cell Development 96%
- Single cell transcriptomics reveal temporal dynamics of critical regulators of germ cell fate during mouse sex determination 94%
- Single-cell transcriptomic profiling of the neonatal oviduct and uterus reveals new insights into upper Müllerian duct regionalization 93%
Similar papers in this journal
- MEIG1/PACRG associated and non-associated functions of axonemal dynein light intermediate polypeptide 1 (DNALI1) in mammalian spermatogenesis 96%
- Testosterone-Induced Metabolic Changes in Seminal Vesicle Epithelial cells Alter Plasma Components to Enhance Sperm Motility 96%
- FBXO24 deletion causes abnormal accumulation of membraneless electron-dense granules in sperm flagella and male infertility 95%
Similar papers in this journal
- MRP5 and MRP9 Play a Concerted Role in Male Reproduction and Mitochondrial Function 96%
- YTHDC2 serves a distinct late role in spermatocytes during germ cell differentiation 95%
- Sub-chronic elevation in ambient temperature drives alterations to the sperm epigenome and accelerates early embryonic development in mice 94%
Similar papers in this journal
- Epididymis-specific RNase A family genes regulate fertility and small RNA processing 95%
- Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activation and sperm motility in mice 95%
- Asynchronous microexon splicing of LSD1 and PHF21A during neurodevelopment 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.