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LKB1 Is Physiologically Required for Sleep from Drosophila to the Mouse

Liu, Z.; Jiang, L.; Li, C.; Li, C.; Yang, J.; Wang, D.; Mao, R.; Rao, Y.

2021-12-14 neuroscience
10.1101/2021.12.13.472078 bioRxiv
Show abstract

LKB1 is known as a master kinase for 14 kinases related to the adenosine monophosphate (AMP)-activated protein kinase (AMPK). Two of them (SIK3 and AMPK) have previously been implicated in sleep regulation. We generated loss-of-function (LOF) mutants for Lkb1 in both Drosophila and mice. Sleep was reduced in Lkb1-mutant flies and in flies with neuronal deletion of Lkb1. Sleep was reduced in mice after virally mediated reduction of Lkb1 in the brain. Electroencephalography (EEG) analysis showed that non-rapid eye movement (NREM) sleep and sleep need were both reduced in Lkb1-mutant mice. These results indicate that LKB1 plays a physiological role in sleep regulation conserved from flies to mice.

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