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Genomic ancestry, diet and microbiomes of Upper Palaeolithic hunter-gatherers from San Teodoro cave (Sicily, Italy)

Scorrano, G.; Nielsen, S. H.; Lo Vetro, D.; Mackie, M.; Margaryan, A.; Fotakis, A. K.; Martinez-Labarga, C.; Fabbri, P. F.; Allentoft, M. E.; Carra, M.; Martini, F.; Rickards, O.; Olsen, J. V.; Cappellini, E.; Sikora, M.

2021-12-08 genetics
10.1101/2021.12.08.471745 bioRxiv
Show abstract

Recent improvements in the analysis of ancient biomolecules from human remains and associated dental calculus have provided new insights into the prehistoric diet and past genetic diversity of our species. Here we present a "multi-omics" study, integrating genomic and proteomic analyses of two post-Last Glacial Maximum (LGM) individuals from San Teodoro cave (Italy), to reconstruct their lifestyle and the post-LGM resettlement of Europe. Our analyses show genetic homogeneity in Sicily during the Palaeolithic, representing a hitherto unknown Italian genetic lineage within the previously identified "Villabruna cluster". We argue that this lineage took refuge in Italy during the LGM, followed by a subsequent spread to central-western Europe. Multi-omics analysis of dental calculus showed a diet rich of animal proteins which is also reflected on the oral microbiome composition. Our results demonstrate the power of using a multi-omics approach in the study of prehistoric human populations.

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