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RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cells

Shi, P.; Murphy, M. R.; Aparicio, A. O.; Kesner, J. S.; Fang, Z.; Chen, Z.; Trehan, A.; Wu, X.

2021-11-30 molecular biology
10.1101/2021.11.30.470032 bioRxiv
Show abstract

While single-cell sequencing has allowed rapid identification of novel cell types or states and associated RNA markers, functional studies remain challenging due to the lack of tools that are able to target specific cells based on these markers. Here we show that targeting a single marker RNA with CRISPR/RfxCas13d led to collateral transcriptome destruction in human cells, which can be harnessed to inhibit cell proliferation or to suppress cell state transition.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.