Matrix-assisted laser desorption ionization time-of-flight mass spectrometry identification of methanogens of clinical interest.
Guindo, C. O.; Amir, L.; Couder, C.; Drancourt, M.; Grine, G.
Show abstract
Methanogens, the archaea uniquely detoxifying fermentative hydrogen into methane in the digestive tract, are increasingly detected in pathology situations, rendering their rapid identification mandatory. We improved the experimental protocol to identify broth-cultured methanogens by matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI- TOF-MS). A database incorporating 34 reference spectra derived from 16 methanogen reference strains representative of eight species, supported further identification of 21 Methanobrevibacter smithii and 14 Methanobrevibacter oralis isolates broth-cultured from human stool and oral fluid, respectively, with scores > 2. In addition, MALDI-TOF-MS differentiated five Methanobrevibacter smithii genotypes incorporated in the study. Data here reported found MALDI-TOF-MS as a first line identification method for methanogens recovered from microbiota and clinical samples.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Description of Klebsiella spallanzanii sp. nov. and of Klebsiella pasteurii sp. nov. 94%
- Comparative analysis of L-fucose utilization and its impact on growth and survival of Campylobacter isolates 94%
- Isolation and comparative genomic analysis of reuterin-producing Lactobacillus reuteri from poultry gastrointestinal tract 93%
Similar papers in this journal
- Highly diverse - low abundance methanogenic communities in hypersaline microbial mats of Guerrero Negro B.C.S., assessed through microcosm experiments 94%
- MinION Sequencing of colorectal cancer tumour microbiomes - a comparison with amplicon-based and RNA-Sequencing 94%
- Rumen Sampling Methods Bias Bacterial Communities Observed 94%
Similar papers in this journal
- Nested PCR to optimize rpoB metabarcoding for low-concentration and host-associated bacterial DNA 95%
- High prevalence of Klebsiella pneumoniae in European food products: a multicentric study comparing culture and molecular detection methods 93%
- Library Preparation and Sequencing Platform Introduce Bias in Metagenomic-Based Characterizations of Microbiomes 93%
Similar papers in this journal
- Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution 95%
- Metagenomics reveals fibre fermentation and AMR pathways in red grouse (Lagopus scotica) microbiota 93%
- Long-term impact of fecal transplantation in healthy volunteers 92%
Similar papers in this journal
- Cultivation-based quantification and identification of bacteria at two hygienic key sides of domestic washing machines 93%
- Exploring the phytobeneficial and biocontrol capacities of endophytic bacteria isolated from hybrid vanilla pods 93%
- Rhizocarpon geographicum lichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.