Identification of Escherichia coli Genes Essential for High-Level, Clinically Relevant, Resistance to Antibiotics
Reyes Fernandez, E. Z.; Alon Cudkowicz, N.; Steiner Mordoch, S.; Schuldiner, S.
Show abstract
Antibiotic resistance is one of the biggest public health challenges of our time. Here we present a novel approach to recognizing molecular mechanisms essential for maintaining high-level clinically relevant antibiotic resistance. To identify essential genes in this context, we subjected Escherichia coli EV18, a strain highly resistant to quinolones, to random transposon mutagenesis. This strains unique advantage is that the screen is performed at very high concentrations of the antibiotic, non-permissive for most strains. The transposons insertion affected the transcription of five genes required for the maintenance of resistance in EV18. Three of these genes (YihO, YhdP, and WaaY) have not been previously identified as essential for high-level antimicrobial resistance (AMR). Our work provides a new perspective to identify and characterize novel players crucial for maintaining AMR in E. coli.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Ceragenins and antimicrobial peptides kill bacteria through distinct mechanisms 96%
- Beyond self-resistance: ABCF ATPase LmrC is a signal-transducing component of an antibiotic-driven signaling cascade hastening the onset of lincomycin biosynthesis 96%
- A decrease in fatty acid synthesis rescues cells with limited peptidoglycan synthesis capacity 96%
Similar papers in this journal
- Lysis cassette-mediated exoprotein release in Yersinia entomophaga is controlled by a PhoB-like regulator 96%
- Gallocin A, an atypical two-peptide bacteriocin with intramolecular disulfide bonds required for activity 96%
- C-di-AMP levels modulate Staphylococcus aureus cell wall thickness as well as virulence and contribute to antibiotic resistance and tolerance 96%
Similar papers in this journal
- Staphylococcus aureus does not synthesize arginine from proline under physiological conditions 97%
- The Gene Encoding Ornithine Decarboxylase for Putrescine Biosynthesis Is Essential for the Viability of Fusobacterium nucleatum 96%
- FrlP, an ABC type I importer component of Bacillus subtilis: regulation and impact in bacterial fitness 96%
Similar papers in this journal
- DNA modifications impact natural transformation of Acinetobacter baumannii 96%
- Correction of non-random mutational biases along a linear bacterial chromosome by the mismatch repair endonuclease NucS 96%
- Staphylococcal aconitase expression during iron deficiency is controlled by an sRNA-driven feedforward loop and moonlighting activity 95%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.