Back

Determining the architecture of nuclear ring of Xenopus laevis nuclear pore complex using integrated approaches

Ren, H.; Tai, L.; Zhu, Y.; Chan, C.; Zhao, Q.; Xu, J.; Wang, X.; Ma, X.; Zhao, L.; Huang, X.; Yin, G.; Jia, M.; Zhu, X.; An, Y.; Zhao, G.; Huang, C.; Zhang, L.; Fan, J.; Sun, F.; Zhang, C.

2021-11-11 molecular biology
10.1101/2021.11.10.468004 bioRxiv
Show abstract

The nuclear pore complexes (NPCs) are large protein assemblies as a physical gate to regulate nucleocytoplasmic transport. Here, using integrated approaches including cryo-electron microscopy, hybrid homology modeling and cell experiment, we determined the architecture of the nuclear ring (NR) from Xenopus laevis oocytes NPC at subnanometer resolution. In addition to the improvement of the Y complex model, eight copies of Nup205 and ELYS were assigned in NR. Nup205 connects the inner and outer Y complexes and contributes to the assembly and stability of the NR. By interacting with both the inner Nup160 and the nuclear envelope (NE), the N-terminal {beta}-propeller and -solenoid domains of ELYS were found to be essential for accurate assembly of the NPC on the NE.

Matching journals

The top 2 journals account for 50% of the predicted probability mass.

50% of probability mass above

"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.