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Fruit flies subjected to TBI exhibit genotype-dependent changes in seizure incidence and duration

Krcmarik, T. R.; Willes, A. M.; Yang, A.; Weber, S. L.; Brusich, D.

2021-10-27 neuroscience
10.1101/2021.10.26.465914 bioRxiv
Show abstract

Traumatic brain injury (TBI) is common and often debilitating. One complication following TBI is post-traumatic seizures (PTS). However, there is a poor understanding of PTS, in part, because it is challenging to model. We sought to develop a fly model of PTS. We used a high-impact trauma (HIT) device to inflict TBI and followed this with behavioral, bang-testing methods to assess seizure behavior. Our results showed PTS incidence was sensitive to genetic background. We also found seizure duration was most pronounced at 30 minutes after injury. Our findings support the efficacy of our fly model for coarse screening of seizure behavior. We expect this model will be useful in first-pass screens intended to identify modifiers of seizure risk following TBI.

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