Lysosomes dock to omegasomes and ER-connected Phagophores During DNAJB12 and GABARAP-Dependent Selective-Autophagy of Misfolded P23H-Rhodopsin
Kennedy, A. S.; Ren, H. Y.; Madden, V.; Cyr, D. M.
Show abstract
We report on how the ER-associated-autophagy pathway (ERAA) delivers P23H-rhodopsin (P23H-R) to the lysosome. P23H-R accumulates in an ERAD-resistant conformation that is stabilized by DNAJB12 and Hsp70. P23H-R, DNAJB12, and FIP200 co-localize in discrete foci that punctuate the rim of omegasome rings coated by WIPI1. P23H-R tubules thread through the wall of WIPI1 rings into their central cavity. Transfer of P23H-R from ER-connected phagophores to lysosomes requires GABARAP, and is associated with the transient docking of lysosomes to WIPI1 rings. Instances of lysosomes docking to WIPI1 foci are constitutive, and increase 250% upon P23H-R expression. After departure from WIPI1 rings, new patches of P23H-R are seen in the membranes of lysosomes. The absence of GABARAP prevents transfer of P23H-R from phagophores to lysosomes without interfering with docking. These data identify lysosome docking to omegasomes as an important step in the DNAJB12 and GABARAP-dependent autophagic disposal of dominantly toxic P23H-R.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- PQLC2 signals lysosomal cationic amino acid abundance to the C9orf72 complex 98%
- V-ATPase is a universal regulator of LC3 associated phagocytosis and non-canonical autophagy 97%
- Autophagy cargo receptors are secreted via extracellular vesicles and particles in response to endolysosomal inhibition or impaired autophagosome maturation 97%
Similar papers in this journal
Similar papers in this journal
- Selective clearance of the inner nuclear membrane protein emerin by vesicular transport during ER stress 97%
- A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress 97%
- The peroxisomal exportomer directly inhibits phosphoactivation of the pexophagy receptor Atg36 to suppress pexophagy in yeast 96%
Similar papers in this journal
- Rhomboid protease RHBDL4 promotes retrotranslocation of aggregation-prone proteins for degradation 97%
- A Trio of Ubiquitin Ligases Sequentially Drive Ubiquitylation and Autophagic Degradation of Dysfunctional Yeast Proteasomes 97%
- ER-embedded UBE2J1/RNF26 ubiquitylation complex in spatio-temporal control of the endolysosomal pathway 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.