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Blind deconvolution in autocorrelation inversion for multi-view light sheet microscopy

Corbetta, E.; Candeo, A.; Bassi, A.; Ancora, D.

2021-10-05 biophysics
10.1101/2021.10.05.463163 bioRxiv
Show abstract

Combining the information coming from multi-view acquisitions is a problem of great interest in light-sheet microscopy. Aligning the views and increasing the resolution of their fusion can be challenging, especially if the setup is not fully calibrated. Here, we tackle these issues by proposing a new reconstruction method based on autocorrelation inversion that avoids alignment procedures. On top of this, we add a blind deconvolution step to improve the resolution of the final reconstruction. Our method permits us to achieve inherently aligned, highly resolved reconstructions while, at the same time, estimating the unknown point-spread function of the system.

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