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Distinct brainstem to spinal cord noradrenergic pathways differentially regulate spinal neuronal activity

Kucharczyk, M. W.; Di Domenico, F.; Bannister, K.

2021-09-26 neuroscience
10.1101/2021.09.25.461801 bioRxiv
Show abstract

Brainstem to spinal cord pathways modulate spinal neuronal activity. We implemented locus coeruleus (LC) targeting strategies by microinjecting CAV-PRS-ChR2 virus in the spinal cord (LC:SC module) or LC (LC:LC module). While activation of both modules inhibited evoked spinal neuronal firing via 1-adrenoceptor-mediated actions, LC:SC opto-activation abolished diffuse noxious inhibitory controls. The LC as a pain generator is likely mechanistically underpinned by maladaptive communication with discrete descending modulatory pathways.

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