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Artificial introns for effective expression of transgenes in mammalian cells

Naberezhnov, D. S.; Kirsanov, K. I.; Lesovaya, E. A.; Yakubovskaya, M. G.

2021-09-01 molecular biology
10.1101/2021.09.01.457939 bioRxiv
Show abstract

Introns are widely used in the assembly of genetic constructions expressing transgenic proteins in eukaryotic cells for the enhancement of this expression. However, the choice of introns that can be applied for such purposes is limited by the excessively large size of the majority of natural introns (several thousand nucleotides) and therefore they cannot be cloned in a genetic construction. With the help of site-directed mutagenesis we have generated a library of short (99 nucleotides long) introns. The efficiency of these introns in the enhancement of gene expression was analyzed. As a result, a set of 12 introns was selected. The generated intros can be used for genetic constructions with high expression level of recombinant proteins.

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