Plasma Contains Ultra-short Single-stranded DNA in Addition to Nucleosomal cfDNA
Cheng, J. C.; Morselli, M.; Huang, W.-L.; Heo, Y. J.; Pinheiro-Ferreira, T.; Li, F.; Wei, F.; Chia, D.; He, H.-J.; Cole, K.; Su, W.-C.; Pellegrini, M.; Wong, D. T.
Show abstract
Plasma cell-free DNA is a widely used biomarker for diagnostic screening. We introduce uscfDNA-seq, a single-stranded cell-free DNA NGS pipeline, which bypasses previous limitations to reveal a novel population of ultrashort single-stranded cell-free DNA in plasma with a modal size of 50nt. This species of cfDNA aligns predominantly to the nuclear genome and could potentially be used for novel biomarker discovery.
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