A nanopore ion source delivers single amino acid and peptide ions directly into the gas phase
Drachman, N.; LePoitevin, M.; Szapary, H.; Wiener, B.; Maulbetsch, W.; Stein, D.
Show abstract
AbstractWe report a nanopore ion source for mass spectrometry that delivers ions directly into high vacuum from aqueous solutions. The ion source comprises a pulled quartz pipette with a sub-100 nm opening. Ions escape an electrified meniscus by ion evaporation and travel along collisionless trajectories to the ion detector. We measure mass spectra of 16 different amino acid ions, post-translationally modified variants of glutathione, and the peptide angiotensin II, showing that these analytes can be emitted as desolvated ions. The emitted current is composed of ions rather than charged droplets, and more than 90% of the current can be recovered in a distant collector. By circumventing the sample loss mechanisms inherent to conventional electrospray ionization (ESI), where charged droplets are sprayed into a background gas that scatters ions and degrades their transmission, the nanopore ion source could enable more sensitive proteomic analyses.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Development of a Platform for High-Resolution Ion Mobility Separations Coupled with Messenger Tagging Infrared Spectroscopy for High-Precision Structural Characterizations 95%
- Patch-Clamp Proteomics of Single Neuronal Somas in Tissue Using Electrophysiology and Subcellular Capillary Electrophoresis Mass Spectrometry 94%
- Contaminant Spot Check and Removal Assay (ContamSPOT) for Mass Spectrometry Analysis 94%
Similar papers in this journal
- Ion mobility mass spectrometry unveils global protein conformations in response to conditions that promote and reverse liquid-liquid phase separation 92%
- High-Voltage Biomolecular Sensing Using a Bacteriophage Portal Protein Covalently Immobilized Within a Solid-State Nanopore 92%
- An RNA to rule them all: Critical steps in Lassa virus ribonucleoparticle assembly and recruitment 91%
Similar papers in this journal
- Gel-assisted mass spectrometry imaging 94%
- Single Cell Proteomics Using a Trapped Ion Mobility Time-of-Flight Mass Spectrometer Provides Insight into the Post-translational Modification Landscape of Individual Human Cells 93%
- Mass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins 93%
Similar papers in this journal
Similar papers in this journal
- Subcellular Mass Spectrometry Reveals Proteome Remodeling in an Asymmetrically Dividing (Frog) Embryonic Stem Cell 94%
- Hyperspectral Oblique Plane Microscopy Enables Spontaneous, Label-Free Imaging of Biological Dynamic Processes in Live Animals 92%
- Accurate Virus Identification with Interpretable Raman Signatures by Machine Learning 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.