Mucin induces CRISPR-Cas defence in an opportunistic pathogen
Almeida, G. M.; Hoikkala, V.; Ravantti, J. J.; Rantanen, N.; Sundberg, L.-R.
Show abstract
Parasitism by bacteriophages has led to the evolution of a variety of defense mechanisms in their host bacteria. However, it is unclear what factors lead to specific defenses being deployed upon phage infection. To explore this question, we exposed the bacterial fish pathogen Flavobacterium columnare to its virulent phage V156 in the presence of a eukaryotic host signal (mucin). All tested conditions led to some level of innate immunity, but the presence of mucin led to a dramatic increase in CRISPR spacer acquisition, especially in low nutrient conditions where over 60% of colonies had obtained at least one new spacer. Additionally, we show that the presence of a competitor bacterium further increases CRISPR spacer acquisition in F. columnare. These results suggest that ecological factors are important in determining defense strategies against phages, and that the concentration of phages on metazoan surfaces may select for the diversification of bacterial immune systems.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Highly diverse flavobacterial phages as mortality factor during North Sea spring blooms 95%
- Unexpectedly high mutation rate of a deep-sea hyperthermophilic anaerobic archaeon 95%
- Genetic and phylogenetic analysis of dissimilatory iodate-reducing bacteria identifies potential niches across the world's oceans 94%
Similar papers in this journal
- Preparation of functional metagenomic libraries from low biomass samples using METa assembly and their application to capture antibiotic resistance genes 94%
- Adaptation of Bacillus thuringiensis to plant colonisation affects differentiation and toxicity 94%
- Farmed oyster mortality follows consistent Vibrio community reorganization 94%
Similar papers in this journal
- Higher-order interactions shape microbial interactions as microbial community complexity increases 94%
- Comprehensive Comparative Genomics Reveals Over 50 Phyla of Free-living and Pathogenic Bacteria are Associated with Diverse Members of the Amoebozoa 93%
- Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model Archaeon 93%
Similar papers in this journal
Similar papers in this journal
- CRISPR-Cas inhibits plasmid transfer and immunizes bacteria against antibiotic resistance acquisition in manure 95%
- Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high density growth 94%
- Prophage-marine diazotroph interplay shapes both biofilm structure and nitrogen release 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.