Selection of reference genes for quantitative analysis of microRNA expression in three cancers.
Veryaskina, Y.; Titov, S. E.; Ivanov, M. K.; Ruzankin, P. S.; Tarasenko, A. S.; Shevchenko, S. P.; Kovynev, I. B.; Stupak, E. V.; Pospelova, T. I.; Zhimulev, I. F.
Show abstract
MicroRNAs (miRNAs) are promising biomarkers in cancer research. Quantitative PCR (qPCR), also known as real-time PCR, is the most frequently used technique for measuring miRNA expression levels. The use of this technique, however, requires that expression data be normalized against reference genes. The problem is that a universal internal control for quantitative analysis of miRNA expression by qPCR has yet to be known. The aim of this work was to find the miRNAs with stable expression in the thyroid gland, brain and bone marrow according to NanoString nCounter miRNA quantification data. As a results, the most stably expressed miRNAs were as follows: miR-361-3p, -151a-3p and -29b-3p in the thyroid gland; miR-15a-5p, -194-5p and -532-5p in the brain; miR-140-5p, -148b-3p and -362-5p in bone marrow; and miR-423-5p, -28-5p and -532-5p, no matter what tissue type. These miRNAs represent promising reference genes for miRNA quantification by qPCR.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Exploring the Diagnostic Potential of miRNA Signatures in the Fabry Disease Serum: A Comparative Study of Automated and Manual Sample Isolations 95%
- Selection of internal references for transcriptomics and RT-qPCR assays in Neurofibromatosis type 1 (NF1) related Schwann cell lines 95%
- The novel microRNAs hsa-miR-nov7 and hsa-miR-nov3 are over-expressed in locally advanced breast cancer 94%
Similar papers in this journal
- Development of a Single Molecule Counting Assay to Differentiate Chromophobe Renal Cancer and Oncocytoma in Clinics 94%
- A novel role for the tumor suppressor gene ITF2 in lung tumorigenesis and chemotherapy response 93%
- Cancer metabolic subtypes and their association with molecular and clinical features 93%
Similar papers in this journal
- Double-targeted knockdown of miR-21 and CXCR4 inhibits malignant glioma progression by suppression of the PI3K/AKT and Raf/MEK/ERK pathways 92%
- Targeting Aryl Hydrocarbon Receptor with small molecule, 1'H-indole-3'-carbonyl -thiazole-4-carboxylic acid methyl ester blocked human glioma cell invasion via MYH9 90%
- Expression of nitric oxide synthase and nitric oxide levels in peripheral blood cells and oxidized low-density lipoprotein levels in saliva as early markers of severe dengue 90%
Similar papers in this journal
Similar papers in this journal
- Structural variability, expression profile and pharmacogenetics properties of TMPRSS2 gene as a potential target for COVID-19 therapy 93%
- Visual Clustering of Transcriptomic Data from Primary and Metastatic Tumors - Dependencies and Novel Pitfalls 93%
- Small RNA Sequencing Reveals a Distinct MicroRNA Signature between Glucocorticoid Responder and Glucocorticoid Non-responder Primary Human Trabecular Meshwork Cells after Dexamethasone Treatment 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.